Friday, 21 February 2020

Perils of processing pitfalls

During the Fall and Winter seasons the IPM lab focuses on processing the numerous field-collected samples. One of these tasks is processing pitfalls. Pitfall traps are used to monitor insect pests, especially those that crawl on the surface of the ground like adult beetles. A pitfall trap consists of a small container filled with RV plumbing anti-freeze submerged into the soil so its opening is level with the soil surface (Fig. 1). A rainfall cover is staked to the ground 1” above the trap to keep rain and larger animals like mice out. The trap is left for seven to 10 days then the content is retrieved, labelled and preserved in ethanol and refrigerated until processed at a later date.
Fig. 1. Pitfall trap deployment consist of filling the container with antifreeze (A), placing the filled inner container into the pre-cut hole in the soil then protecting the opening with a rain cover (B), to produce a submerged pitfall trap whose opening is flush with the surrounding soil surface (C). Photos and diagram by Shelby Dufton.

During the Fall and Winter months, stored pitfalls are retrieved for individual processing via submersion in water. The samples, which are stored in cheesecloth bundles, are opened into a tray of water (~1.5 L water in a ~4 L tray) because arthropods normally float and most of the soil/rocks sink. This pre-sorts the sample. Elaterids, carabids, staphylinids, and arachnids are then hand-sorted, recorded, and transferred to labelled vials with 95% ETOH. They are stored for further processing, like sex determination and species identification.

Over the past month, Tia and I have been working on two main studies using pitfalls, the Canola Agronomic Research Program (CARP) project and the wireworm monitoring project, both of which are extensive projects with sites across the Peace River region. The wireworm project additionally involves testing pheromone lures used in pitfall trapping.
Fig. 2. Pitfall trap processing involves submersion of a muddy wireworm monitoring sample in a 38 cm by 23 cm tub containing ~1.5 litres of water (A) and carefully retrieving the many beetles and grasshoppers from the sample (B). 

It is important to monitor wireworms, which are actually the larvae of click beetles (Elataridae), because they can be harmful to crops throughout their lifecycle. Click beetle larvae, often referred to as wireworms, cause economic damage as they feed on germinating seeds and decrease both plant establishment and densities in all field crops. Pheromone lures attract wireworms by emitting the same chemicals as germinating seeds, which the wireworms detect when finding food. Pitfall traps mainly catch adult click beetles because the larvae live beneath the soil surface. For more information on how to check your field for wireworms, refer to the PPMN blog and field guide.

We also monitor carabid beetles retrieved from these pitfall samples. Carabids are important to the agroecosystem because this large group of species can be natural predators of click beetles. At least 16 species of carabids are commonly found in fields in the Peace River region. They range in size and color (Fig. 3).
Fig. 3. A selection of pinned Poecilus lucublandus (Coleoptera: Carabidae) from a previous project conducted at AAFC-Beaverlodge demonstrates the diversity in size (7-11 mm) and color (dark, iridescent greens, blues, and purples that can be much brighter in life than in pictures) even within one species. 

References: 
Philip, H. 2015.  Field crop and forage pests and their natural enemies in western Canada: identification and management field guide. Agriculture and Agri-Food Canada, Saskatoon, SK. Access the free PDF.

Friday, 14 February 2020

Meet our students - Introducing Tia!

Hello! My name is Tia Malloff and I am one of the new co-op students in the Insect Pest Management program at the Beaverlodge Research Farm. I will be working the IPM for an 8-month term!

I am a fourth-year student at the University of British Columbia studying Chemical Biology.  After spending my last co-op term in the chemistry industry in California, I decided to move to the Peace River region to focus more on biology. I am looking forward to learning more about the agroecosystem of the Peace.

Growing up in the Kootenays and helping out on my grandparents’ farms has given me a great appreciation for both agriculture and the outdoors. While these farms paled in comparison to many of the farms here in northern Alberta, they instilled in me the importance of insects in agriculture.



The Peace River region is the largest producer of creeping red fescue in North America. The seed is used to grow forage, in land reclamation, and is often a filler in turf seed products. I am currently counting the seed-bearing stems found in each plant bunch (pictured above). Once counted, the stems will be threshed in order to calculate the yield of seeds per stem and per plant.

I am excited for what the next 8 months will bring and thrilled with the opportunity to explore the Peace!

Meet our students - Introducing Isaac!

Hello, my name is Isaac and I am a new Co-op student in the IPM lab at the Beaverlodge Research Farm. I will be working at the farm for both the winter and summer terms!

I am currently in my third year at the University of Manitoba, where I am working towards a Bachelor of Science in Evolutionary Biology and Biodiversity, as well as a Bachelor of Arts in History. Weird combination, I know! I am excited to learn more about real-world applications of entomology and its relation to agriculture.

This week my fellow Co-op student Tia and I have been processing data from the previous field season. These data have mostly come in the form of pitfalls. We identify and sort out Elaterid (click), Carabid (ground), and Staphylinid (rove) beetles from the rest of the critters—like spiders, flies, and moths caught in the traps. We track the number of adults and larvae in each group, then place the beetles in vials filled with ethanol that will await further processing.


I am looking forward to learning more about insect pest management and lab work this winter. In the summer, I look forward to being able to go out in the field as well as getting to explore Alberta and British Columbia!